Coenzyme Q10 Supplementation Effects on In Vitro Maturation, Fertilization, and Early Embryonic Development in Pigs

Caitlin Streacker, Brian D. Whitaker

Abstract


The objective of this study was to determine the reduction of polyspermic penetration, and increase of mitochondrial activity, in early pig embryonic development by supplementing different concentrations of coenzyme Q10 during oocyte maturation. Oocytes (n = 1,100) were supplemented during the last 24 h of maturation with 0 (control), 10, 50, or 100 μM of coenzyme Q10. After in vitro fertilization (IVF), embryos were evaluated for fertilization kinetics (penetration, polyspermic penetration, male pronuclear formation), and subsequent embryonic development and mitochondrial activity. Supplementation of 100 μM coenzyme Q10 was detrimental to the oocytes, as they had significantly lower (p < 0.05) fertilization kinetic and early embryonic development rates to the other treatment groups. There were no differences in fertilization kinetic and early embryonic development rates between the 0, 10 and 50 μM coenzyme Q10 treatment groups. Oocytes, matured in medium supplemented with 50 μM coenzyme Q10, ultimately developed into embryos with a significantly greater (p < 0.05) presence of intact mitochondrial membranes (observed at both 48 and 144 h post-IVF) compared to oocytes not supplemented with coenzyme Q10. In summary, supplementation of 100 μM coenzyme Q10 during oocyte maturation is detrimental, yet supplementation of 50 μM coenzyme Q10 leads to a higher occurrence of intact mitochondrial membranes in the in vitro produced pig embryos.


Keywords


Coenzyme Q10; in vitro fertilization; polyspermy; mitochondria; pigs

Full Text:

PDF


DOI: http://dx.doi.org/10.18061/ojs.v119i2.6366

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Caitlin Streacker, Brian D. Whitaker

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

 


Visit The Ohio Academy of Science's webpage

Visit our Knowledge Bank site for articles published from 1900 through 2013.

The Ohio Journal of Science is published by The Ohio State University Libraries in partnership with The Ohio Academy of Science.

If you encounter problems with the site or have comments to offer, including any access difficulty due to incompatibility with adaptive technology, please contact libkbhelp@lists.osu.edu.

ISSN: 2471-9390 (Online); 0030-0950 (Print)