Mutagenic Potential of Artificial Athletic Field Crumb Rubber at Increased Temperatures

Authors

  • Michael J. Dorsey Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Elizabeth Anderson Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Oliva Ardo Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Max Chou Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Edward Farrow Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Ethan L. Glassman Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Margaret Manley Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Herbert Meisner Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Cecily Meyers Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Nolan Morley Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Joseph Rominger Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Madison Sena Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Madison R. Stiefbold Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Benjamin Stites Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Maxwell Tash Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Emma Weber Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215
  • Patrick E. Counts Wyoming High School 106 Pendery Ave. Cincinnati, OH 45215

DOI:

https://doi.org/10.18061/ojs.v115i2.4857

Keywords:

artificial athletic fields, mutagenic, temperature, crumb rubber, fluctuation assay

Abstract

Rubber tires contain several compounds that are known or suspected carcinogens.  Many carcinogens are mutagens, and fluctuation assays based on the Ames test can be used as an initial screen for mutagenic potential.  Granulated crumb rubber from recycled tires is commonly used in the creation of artificial athletic fields, and the surface temperature of these fields can reach levels far above the ambient temperature.  In this study, crumb rubber samples taken directly from four separate artificial athletic field surfaces were used to make leachates using water at different temperatures.  For each of these fields, leachates obtained in water at 70 ºC showed significant mutagenic potential (p ≤ .001) in Salmonella typhimurium fluctuation assays.  Leachates obtained in water at 40 ºC showed no mutagenic potential for any of the fields tested.  For one field, crumb rubber heated in water at temperatures as low as 50 ºC resulted in significant mutagenic potential (p ≤ 0.001).  Water used in an experiment designed to mimic the irrigation of an artificial athletic field also showed mutagenic potential (p ≤ 0.001) in a fluctuation assay.  These results suggest that at the higher temperatures that can exist on artificial athletic field surfaces, the crumb rubber infill on these artificial athletic fields can become the source of a water soluble agent with mutagenic potential in bacteria.

Additional Files

Published

2015-08-04

Issue

Section

Articles